Spectrophotometer Concentration and Standard Curve Essay

Lab Report 4 Biology 103 Prof. Dr. Johnson Spectrophotometer; the finding of protein concentration of an unknown sample of BSA, and by using the standard curve. Abstract A spectrophotometer’s purpose is to use colors of the light spectrum to determine the concentration of light absorbing molecules in a solution. (p.59) In this particular lab, our mission was to determine the protein concentration and the standard curve of the unknown sample of BSA. This, by preparing five dilutions of the unknown solution of BSA together with other known concentrations, and then experimenting by observing how the concentrations were passed through the spectrophotometer. The outcome resolved in the absorption levels being decreased, and this†¦show more content†¦The same solution of 0.5 ml BSA was then added from test tube 1 to the test tube 2 after being properly mixed, and from test tube 2 the solution was being added to test tube 3, and so forth all the way up to test tube 5, with the same exact procedure. From the last tube, we then disposed the 0.5 ml solution. After above procedures, we now labeled another test tube â€Å"blank†; 0.5 ml blank distilled water was purred into the tube with the serial dilution of 1:10. We also had a tube C labeled â€Å"unknown† with the same 0.5 ml of solution. And after adding 5ml of Coomassie Blue to each tube (1-5) and to the blank, the result of absorbance was read at 595 nm. Result | Protein Concentration( µg/ml) | Absorbance(595 nm) | Tube 1 | 120 | .922 | Tube 2 | 60 | .557 | Tube 3 | 30 | .377 | Tube 4 | 15 | .180 | Tube 5 | 7.5 | .082 | Unknown: .759 | The ending result of this experiment confirms that as five test tubes are lined up with the varying level of absorbance, different results in the level of absorbance will appear as well, this is visible in above table. Thus, this is due to the varying amount of water in the solution. The blank sample had a 0.30 in its level of absorbance. 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Analysis Of The Movie The War Against Bullying

Deniz Hernandez Dr. Ball TH 4398 B April 2, 2015 â€Å"The War against Bullying† I. Introduction It started with the name-calling. We had just moved to Houston from California and I was about to begin the third grade at Thompson Elementary. I was â€Å"the new girl!† The â€Å"tall† girl with an â€Å"accent† that nobody wanted to be friends with. I was the â€Å"odd and ugly† girl in the third grade. It was a group of six boys and girls that decided to make my year miserable by bullying me. I never hated school so much. In fact, I remember frequently lying to my parents about being sick because, I did not want to attend school. However, the verbal and emotional abuse continued and I had decided to remain silent. According to the National Center for Education†¦show more content†¦Ã¢â‚¬Å"Its effects on victims, perpetrators, and even bystanders are both immediate and long term and can affect the development and functioning of individuals across generations.†(Donegan 2). What follows after this, will emphasize on the types of bullying, define the problem and its negative impact on students, and discuses nonviolent alternatives to address the matter. II. Definition of Bullying and Its effects According to research by the U.S. Department of Health Human Services, bullying is consider being a form of violence in schools and consists of two people, a bully or intimidator and a victim. â€Å"Bullying is unwanted, aggressive behavior among school aged children that involves a real or perceived power imbalance†¦the behavior is repeated†¦or has the potential to be repeated, over time†¦Ã¢â‚¬  (â€Å"www.stopbullying.gov†) It includes direct or indirect actions such as hitting, verbally assaulting face-to-face, etc. or starting rumors and gossip with the intentions of ruining the victims’ self-esteem and gaining a sense of superiority and power over him or her. Research shows that bullying has a negative impact on the following: the target, the bully, bystanders, and the school environment. In fact, many victims of bullying experience depression, anxiety, feelings of loneliness and isolation, disconnection from school and lose interest in school. In addition, they have a higher risk of substance abuse and in extreme cases,

Euro Crisis Stand off Between France and Germany

Question: What have been the main roles performed by the International Monetary Fund and the European Central Bank in resolving the financial crisis experienced by Greece in recent years? Answer: Introduction The major economic system in the world are going through a phase of turmoil with rising debt crisis. The economy of Greece has been experiencing crisis since the year 2009 which has had serious implications on the government debt sustainability which has increased from 103% of GDP to soaring over 170% of GDP, contracting the economy by 25% , raising unemployment to 25% and making the banking system unstable. (Bulmer, 2014)The Eurozone governments, International Monetary Fund(IMF) and European Central Bank (ECB) has adopted and implied a number of policies alongside measures in order to end the crisis. The crisis in Greece has affected other member states in the Eurozone as they share a common currency, monetary policy and controls fiscal and banking policies.(Blanchard, 2014) The report is a comprehensive reflection of the various endeavors that have been undertaken by International Monetary Fund (IMF) and European Central Bank (ECB) in resolving the financial crisis that Greece is experiencing in the recent period. IMF has 189 participating countries from around the world whereas ECB consists of the European countries that have agreed to the Eurozone agreement. The report deals with the financial assistance and mentoring help that IMF and ECB extended to Greece for a comprehensive bail out to emerge out of its crisis situation. The crisis in Greece started to grow since the 1990s when the government of the country adopted Euro as its national currency which resulted in lower borrowing costs. (Mitsopoulos, 2011)As Greeces policies were controlled by European Central Bank (ECB) rules and regulations, investors trusted the economy and started lending money. Majority of the funds were utilized for serving government spending, compensate for lower tax earning s. IMF and ECB have extended several financial assistance packages to the country to offset its current debt situation as discussed below.(Abboushi, 2011) Role of IMF ECB in Greece crisis Greece crisis was revealed in 2009 when the newly elected government revealed the budgetary deficits of the country, which distanced the country from capital markets as investors started growing uncertain regarding Greeces financial position. Greece position impacted other countries in the Eurozone as investors were hesitant regarding the sustainability of their public finances. Threatening the European banking system as a whole with slowed economic progress and increased unemployment, International Monetary Fund and European Central Bank extended benefits in the light of crisis.(Matsaganis, Social policy in hard times: The case of Greece. Critical Social Policy, 2012) In 2010 and 2012 IMF and other Eurozone government extended two financial package totaling to Euro 240billion. Greece utilized the fund for fiscal and structural adjustments that changed budget deficit from 9.9% of GDP to a surplus of 1.5% in 2014.(De Grauwe, What kind of governance for the eurozone?. , 2010) ECB responded to the Greece crisis by purchasing or pledging to purchase bonds in secondary market through Securities Market Program (SMP) and the Outright Monetary Transactions program (OMT). ECB had forwarded Euro 1 trillion through three-year, low cost loans or long-term refinancing operations (LTRO) into 800 banks in Eurozone. ECBs contributions has been greatly considered important in stabilizing Eurozone especially financial markets and in reducing crisis. ECB extended several purchases through the money market instruments available and also extended mentoring of the financial situation to help Greece bailout of its current economic crisis. However Germany was opposed to the idea of extending help to Greece. Greece a member of Eurozone was feared to spread its crisis to the other European countries. IMF major shareholder being USA supported Greece and extended financial assistance package and also regular mentoring to help Greece bailout of its current situation.(Roubini, 2010) Greece upon receiving the first financial assistance failed to adopt economic reforms related to taxes, fiscal targets, pensions and debt levels. The government highlighted that the first assistance was utilized in paying off most of the past debt payments, upon being deferring payments to creditors. The country called for disbursing the second financial assistance Euro 7.2 billion as it altered on its payments to the IMF in 2015 Euro 1.5 billion. Hence IMF along with other Eurozone countries agreed on extending the third financial assistance package. IMF hence provided a Euro 30 billion under Stand-By-Arrangement(SBA) through its standard lending instruments.(De Santis, 2012) Greece had several governments change during the period of crisis which led to inappropriate applications of required reforms in the country. (Stuckler, 2012) The agreement that IMF and ECB made to finance Greece with Euro 110 Billion financial package along with certain reforms that the country had to make. IMF will analyze the progress of Greece on its assistance on quarterly basis. The following reforms were demanded from Greece in exchange for the assistance. (Bohn, The 2010 euro crisis stand-off between France and Germany: leadership styles and political culture., 2011) Reduce Debt: Greece was continuously unable to repay its loans to IMF and ECB , there needed to be reforms made in its policy. The allocation of the funds of the financial assistance needed to be applied appropriately for it be able to repay back its loans in time. The agreement was made in the light that Greece would be able to tackle its debts and start a growth trajectory within the country. IMF and ECB was deeply interested in seeing the procedure Greece embraced in terms of budgetary cuts, freezing wages and pension for three consecutive years and increase tax. (Blanchard, 2014)These measures would directly address Greeces fiscal and debt issues and will enable the economy utilize the financial assistance for deep reforms to strengthen the economy and put it back on growth tracks. The Greek Prime Minister George Papandreou as well as European Union Economic and Monetary Affairs Commissioner Olli Rehn and IMF Managing Director Dominique Strauss-Kahn agreed that massive measures w ere adopted to prevent further crisis and gain control over Greeces public finances which will eventually modernize the Greek economy. IMF extended these measures as a means for Greece to overcome its current crisis levels. Greece could only reduce its burden of debt by repaying off its previous loans with proper financial planning of its fiscals.(Janssen, 2010) In order to make Greeces government finances sustainable and reducing its fiscal deficits the debt to GDP ratio needs to be on a downward trending. The wages and social benefits is 75 % of total public spending in Greece. The government will curtail any programs that extend social security benefits but will continue to provide benefit to the vulnerable.(MLA, 2011) The pension program will discourage early retirement and encourage to work for more number of years. (Matsaganis, The welfare state and the crisis: the case of Greece, 2011).The government also proposed to curb military expenditure in the period. To improve current business climate: IMF and ECB implied that Greece adopting the measures suggested by them in the international front would not only allow the economy recover from the past but also improve its overall business climate. Though IMF recognizes that the initial period will be stressful for Greece but the fiscal measures reforms would help make the economy more dynamic and robust.(Lattemann, 2008) The measures suggested by IMF and ECB will help Greece be on a growth trajectory, create more jobs and an environment for future sustainability. IMF further suggested that Greece adopt steps to reform its current labor market and labor market policies that will enable it to generate better incomes, will attract investment in state enterprises improving the overall business environment. Foreign investors have become hesitant about investing into Greece companies and state control enterprises but adopting suitable reforms and lowering overall salaries in the labor market will at tract those investors. (Mitsopoulos, 2011)These increased numbers of businesses in the country will generate jobs and pay taxes which will create earnings for the government. The government of Greece was also instructed by IMF and ECB to reform policies in regards to waste as that would greatly impact utility from products and reduce wastes.(Matsaganis, Social policy in hard times: The case of Greece. Critical Social Policy, 2012) Greece needed to also adopt stringent measures to fight corruption by elimination non-transparent procuring practices. IMF along with ECB wanted Greece to improve the existing business environment including political, technological, social changes in the country. (Mamadouh, 2011)IMF and ECB indicated that the environment for conducting business in Greece was not appropriate as people tend to retire early and enjoy pension benefits also the bodies aimed to curb the several benefits the governments extends to the people on summer, Christmas, Easter bonuses . This social reform was meant to encourage people to work more and would d also encourage several foreign companies to set up their facilities in Greece. (Douzinas) Accept Measures: Greece would accept any measures that IMF Managing Director and European countries extend. According to direction given by IMF and ECB Greek governmental authorities started consolidate fiscal measures to 5% of GDP and accept legislative actions. (De Santis, 2012)Great emphasis was made to provide fair measures within the economy that included protection of the most vulnerable. The measure stress of IMF was put on tackling twin issues within Greece of debt and competitiveness.(Visvizi, 2012). IMF and ECB identified through their constant monitoring of the Greece economy that the impounding debt and financial crisis in Greece was majorly allocated to its inability to accept measures laid down by them and incapability within its economy to accept modernized measures. IMF and ECB both proposed at modernization of the Greece economy in the light of the crisis that was overhauling its economy. But earlier governments had failed to assess the graveness of the situation and the results that could come from Greece not reforming its policies. Earlier government had used the recurring financial help to repay off its existing debts, interests and to fund government expenditures. In the final assistance package extended by IMF it was agreed that Greece will have to accept the remedial measures as suggested on its current economic status and accept the necessary implications that arise from applying such measures. Making Greece economy competitive : Greece would adopt measures to reform its policies to these waste reduction, reduce tax evasion and provide protection to the poor. IMF proposed that Greece needed to make its economy more competitive in order to make it pro-growth which implied that policies and reforms that were traditional in nature needed to be modernized. (De Grauwe, What kind of governance for the eurozone?. , 2010)Modernization of the Greek economy will open up opportunities for all, indigenous as well as foreign investors. Competitiveness measures as indicated by IMF directed at cost controlling and reduction of inflation such that Greece could regain control over prices and ensure price competitiveness. (Knight, 2015) Greece was at a budgetary deficit of 13.6% of GDP with public debt mounting to 115% of GDP in 2009. The Greece government in response to IMF indications aimed to consolidate fiscal deficits to 11% of GDP and designed adjustments such that general government deficit rounds of to 3% by 2014 as against 13.6% in the year 2011. (Wyplosz, 2012)Greece economy was functioning at the tail end of Eurozone countries prior to its crisis period. This further made the crisis financially and economically to settle in Greece easily.(Chamley, 2011) The governmental spending measures will be targeted to yield savings of 51/4% of GDP throughout 2013. The government also aimed to freeze pension and wages for three years and abolish payment during Christmas, Easter and summer bonuses while accommodating the lowest income group.(Matsaganis, Social policy in hard times: The case of Greece. Critical Social Policy, 2012) The financial markets in Greece especially the debt market major restructuring. Lenders and international investors were weary regarding Greece financial markets capability to deliver. IMF and ECB wanted Greece to increase its ratings in financial markets such that it could attract investments.(Mantanika, 2011) The major governmental revenues raised through value-added tax, taxes on luxury items, tobacco, alcohol and so on will raise a 4% of GDP in 2013 alone. The government will administer a stringent measure to collect taxes from individuals especially those susceptible to tax evasion.(De Santis, 2012) The tax system was also meant to safeguard revenue from large taxpayers. The budgetary controls were also regarded as an important step in controlling deficits and reduce burdens of debt. The net gain from administering structural reforms was expected to be 1.8% of GDP in the program period.(Lapavitsas, 2012) A Financial Stability Fund was created from the external sources of financing received to ensure proper bank equity and avoid misappropriation of funds. Previous governments in the beginning of the crisis period had misappropriated the funds meant for the country bailing out of the crisis period. This led apprehension in the mind of ECB as well as IMF in regards t its capabilities to repay off dents. Hence IMF on its quarterly monitoring indicated that the country devise a mechanism whereby the financial assistances can be redirected to yield much better results for the country.(Bohn, The 2010 euro crisis stand-off between France and Germany: leadership styles and political culture. , 2011) Conclusions Greece has been gradually recovering from its state of crisis with the several aids that it has received. The financial aid that has been forwarded by IMF and ECB in times of financial breakdown helped the economy regain back its economy and keep the basics functioning. However the most valuable input to the country had been the policies and propositions that IMF along with ECB suggested to help restructure its economy. (De Santis, 2012)Though majority of the steps came at considerable costs and raised speculations regarding the economies stability and viability. Yet the country has been able to bail out of its extreme crisis situation but is yet to get back on its growth trajectory. (De Grauwe, Greece is solvent but illiquid: What should the ECB do? (No. 10694), 2015) The IMF fears while forwarding financial assistance to Greece had been related to the countrys capability in accepting and adopting the major economic reforms. The Eurozone had feared that the countrys crisis could spread to other countries in the Europe that extended help. As Eurozone is bounded by common currency, monetary, banking and fiscal policies, they were apprehensive that extending help to Greece could easily have an effect on the Euro currency rates and raise levels of inflation. The role of IMF had been significant when considering the totality of the situation in which Greece was entangled and from where the country has recovered. (Stuckler, 2012)The IMF major shareholder USA had been very positive about Greece gaining back but was refuted several times as Greece failed initially to adopt the measures suggested by IMF Director. The Greece economy is a major economy in the Eurozone but Germany had severely opposed to IMF endeavors to help Greece bail out. IMF had failed t o assess the debt sustainability of Greece as most of its loans gets extended. Though the costs of financial assistance from IMF is quite high yet it has overlooked all Greeces fallouts and extended loan repayments till 2040 until which Greece gets time to reform and deliver.(Bohn, The 2010 euro crisis stand-off between France and Germany: leadership styles and political culture. , 2011) References Abboushi, S. (2011). Analysis and outlook of the Greek Financial Crisis. Journal of Global Business Management, 7(1), 1. Blanchard, O. J. (2014). Labor market policies and IMF advice in advanced economies during the Great Recession. IZA Journal of Labor Policy, 3(1), 1-23. Bohn, F. . (2011). The 2010 euro crisis stand-off between France and Germany: leadership styles and political culture. International Economics and Economic Policy, 8(1), 7-14. Bohn, F. . (2011). The 2010 euro crisis stand-off between France and Germany: leadership styles and political culture. . Economics and Economic Policy, 8(1), 7-14. Bulmer, S. (2014). Germany and the eurozone crisis: between hegemony and domestic politics. West European Politics, 37(6), 1244-1263. Chamley, C. P. (2011). Why official bailouts tend not to work: an example motivated by Greece 2010. The Economists' Voice, 8(1). De Grauwe, P. (2010). What kind of governance for the eurozone?. . CEPS Policy Brief, (214). De Grauwe, P. (2015). Greece is solvent but illiquid: What should the ECB do? (No. 10694). Centre for European Policy Studies. Douzinas, C. (n.d.). Philosophy and Resistance in the Crisis: Greece and the Future of Europe. 2013: John Wiley Sons. Janssen, R. (2010). Greece and the IMF: Who Exactly is being Saved?. Cent. for Econ. and Policy Res,, 1611. Knight, D. M. (2015). History, time, and economic crisis in central Greece. Palgrave Macmillan. Lapavitsas, C. (2012). Crisis in the Eurozone. Verso Books. Lattemann, S. . (2008). Environmental impact and impact assessment of seawater desalination. Desalination, 220(1), 1-15. Mamadouh, V. . (2011). Financial, Monetary and governance crisis: an outlook on the euro (zone). Tijdschrift voor economische en sociale geografie, 102(1), 111-118. Mantanika, R. . (2011). The spatiality of a social struggle in Greece at the time of the IMF: Reflections on the 2011 mass migrant hunger strike in Athens. City. 15(3-4), 482-490. Matsaganis, M. (2011). The welfare state and the crisis: the case of Greece. Journal of European Social Policy, 501-512. Matsaganis, M. (2012). Social policy in hard times: The case of Greece. Critical Social Policy. 0261018312444417. Mitsopoulos, M. . (2011). Understanding the crisis in Greece: from boom to bust. Palgrave Macmillan. MLA. (2011). Analysis and outlook of the Greek Financial Crisis. Journal of Global Business Management, 7(1), 1.Roubini, N. M. (2010). Crisis economics. Penguin Audio. Stuckler, D. . (2012). There is an alternative: public health professionals must not remain silent at a time of financial crisis. European Journal of Public Health, 22(1), 2-3. Visvizi, A. (2012). The crisis in Greece and the EU-IMF rescue package: Determinants and pitfalls. Acta oeconomica, 62(1), 15-39. Wyplosz, C. (2012). The ECBs trillion euro bet. VoxEU, February (www. voxeu. org/article/ecb-s-trillion-euro-bet). Disappearig government bond spreads in the eurozone back to normal

The Wolf of Wall Street The Rich Life Essays - Film, Finance

The Wolf of Wall Street: The Rich Life What is the secret to becoming filthy rich in the stock market in the modern age? In just under forty years, Apple, a company which began trading publicly in 1980, has created more than $1 trillion in net wealth for investors.The previous chart-topper. Warren Buffett once said " The stock market is a device for transferring money from the impatient to the patient." Warren, Buffett. WiseGeek defines net wealth as ' the total value of assets owned by an individual, company, or other type of organization, less any current liabilities. The goal of most businesses and households alike is to generate a positive net wealth, meaning that the total value of asset surpasses the total amount of debt that is currently owed."' The movie known as The Wolf of Wall Street, directed by Martin Scorsese, a drama/biography defines their American dream as not about becoming middle children and having your kids go to college, but is about becoming ridiculously rich. But the institutions that al low people to become ridiculously rich (wall street bank etc) are not open to people with the wrong background and without the correct connections and breeding. So in light of that contradiction, the only way for normals to achieve the American dream is to find something that is in a grey area of the law . I gave the movie a 5 star simply because it was motivational, inspirational and informative. I thought this movie would be boring at first because it has to do with the stock market and Wall Street which basically deal with numbers all day, long but after I watched the movie, my way of thinking completely changed. Haris 2 The Wolf of Wall Street includes talented and phenomenal actors and actresses. Leonardo DiCaprio, the main character, performed as Jordan Belfort, a american dreamer Which chases a desire of becoming world's most powerful and rich individual with his friends that he helped get rich. Margot Robbie performed as Naomi Lapaglia, a beautiful girl from Dutch which creates a personal bond with the main character and marry with him later on movie. Jonah Hill performed as Donnie Azoff, a middle class worker lives in the same building that Jordan Belfort does. Azoff finds himself confronting Jordan Belfort in shop which is the moment his life changes from a middle class worker to an independent higher class individual. Jon Bernthal performed as Brad, a drug dealer which becomes a close partner with Jordan Belfort after the main character decides to create of his own firm to become financially independent. The movie begins by describing Belfort's first day as a junior stockbroker on Wall S treet. Surrounded by power play ers who live to be rich, get richer or die trying. Jordan has the opportunity to watch and learn firsthand the rules of the game. Little did he know he was stepping into a world where drugs, alcohol, corruption and prostitution are daily occurrences. A few years later, Jordan, who has been dreaming of truly becoming a successful stockbroker in his own right, has left Wall Street and has decided to start his own firm: Stratton Oakmont (SO) in Long Island, New York. Not only is he making millions of dollars daily and illegally at SO, but his firm and wild stockbrokers are the pure definition of debauchery; parties and sexual acts performed with hookers in the office, illegal pills and cocaine - to name but a few - can be found in abundance. This is an outrageous story of a man who had it all, i.e., money, status, prestige, a wife and children, yet who could not help wanting more. A man whose inexcusable greed led him to rob millions of innocent Amer icans so as to enable him to Haris 3 continue in this life of abuse, excesses, perversions and self-destructing behavior. Until he got caught. Jordan Belfort is very successful in his effort at becoming rich. His way of thinking or his mindset were not surely not always effective but they eventually came to be very major in his path of success and they did pay off at the end. Jordan Belfort kept thriving for money even